Vinblastine sulfate isolated from the vinca has been widely used as an anticancer drug. Vinblastine sulfate inhibits the formation of microtubules which are related to cytoskeleton, transport of molecules and cellular polarity. As a result, it inhibits the mitosis and brings to cellular necrosis.
The author undertook the present study to investigate the effect of binblastine sulfate on phosphatase activity in the mouse liver. Albino mice, DDY strain, weighing 2gm were used as experimental animals. The experimental animals were sacrificed at 6, 12, 24, 36 and 48 hours after administration of 12 mg/kg of vinblastine sulfate. The activity of alkaline phosphatase was observed by Gomori´s method and the activity of adenosine triphosphatase was observed by the Wachstein-Meisel´s method for histochemical study.
The results obtained were as follows:
1. The activity of alkaline phosphatase was slightly increased in the central zone and decreased in the intermediate and periportal zone of the hepatic lobule of the 24 and 36 hours vinblastine sulfate treated groups. In 48 hour-vinblastine sulfate treated group, the activity of alkaline phosphatase was recovered to normal.
2. The activity of adenosine triphosphatase was slightly increased in the hepatic lobule of the 12 and 24 hours vinblastine sulfate treated groups but decreased in the 36 and 48 hour vinblastine sulfate treated groups.
Consequently, it is suggested that vinblastine sulfate causes to decrease the activity of alkaline phosphatase and adenosine phosphatase in mouse liver
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